myelogenous leukemia aml  (DSMZ)

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: The characterization of two kinds of iron nanoparticles and their effects on KG1a and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Zeta Potential Analyzer, Expressing

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparisons of differentially expressed genes (DEGs) in two Leukemia cells treated with two kinds of iron nanoparticles: ( A ) number of upregulated and downregulated DEGs (fold change > 1.0) in the PBNP- and FeNP-treated HL60 and KG1a cells and their relationship, ( B ) common DEGs (fold change > 1.5) in a cell treated by two kinds of nanoparticles, ( C ) common DEGs (fold change > 1.5) in two cells treated by a nanoparticle, ( D ) common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles, and ( E ) schematic of cellular functions of common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles. The detailed information of all DEGs is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Top 20 GO terms of the KG1a cells treated with two kinds of iron nanoparticles: ( A , B ) heatmap ( A ) and enrichment network ( B ) colored by the same cluster of GO terms in the FeNP-treated KG1a cells, and ( C , D ) heatmap ( C ) and enrichment network ( D ) colored by the same cluster of GO terms in the PBNP-treated KG1a cells. The colored labels followed the order of top 20 GO terms. ( E ) Venn analysis of top 20 GO terms in the KG1a cells treated with PBNPs and FeNPs. The detailed information of all GO terms is shown in .

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of lipid metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Membrane, Activity Assay

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of metal ion metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Activity Assay, Clinical Proteomics, Membrane

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparative Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs ( p < 0.05): ( A ) comparative top 10 enriched pathways of HL60 and KG1a treated with FeNPs and ( B ) comparative top 10 enriched pathways of HL60 and KG1a treated with PBNPs. The pathways in black and blue were HL60 and KG1a cells, respectively. ( C ) Four-way Venn analysis of all KEGG pathways in four groups. The detailed information of all KEGG pathways is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Validation of RNA-Seq DEGs using RT-qPCR: ( A , B ) the expression levels of 10 selected DEGs in the iron nanoparticle-treated HL60 ( A ) and KG1a ( B ) cells. All values are mean ± SD with n = 3. ns, no significance; *, p < 0.05; **, p < 0.01, ***, p < 0.001, ****, and p < 0.0001. ( C , D ) The comparison of fold change detected by RT-qPCR and RNA-Seq in the iron nanoparticle-treated HL60 and ( C , D ) KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Biomarker Discovery, RNA Sequencing, Quantitative RT-PCR, Expressing, Comparison

Journal: Molecules

Article Title: Peruvoside, a Cardiac Glycoside, Induces Primitive Myeloid Leukemia Cell Death

doi: 10.3390/molecules21040534

Figure Lengend Snippet: Figure 1. Peruvoside is more effective in suppressing the growth of human leukemia cells. (a) Structure of Peruvoside, Digitoxin and Ouabain; (b) Dose response of three different cardiac glycosides (CGs) in human leukemia cell line KG1a and K562 at two different time points. MTT assay was used to assess cell viability 24 and 48 h after treatment (n = 3); (c) IC50 values of different CGs 24 and 48 h after treatment (n = 3).

Article Snippet: Human chronic myelogenous leukemia (CML) K562 cell line and human acute myelogenous leukemia (AML) KG1a cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: MTT Assay

Journal: Molecules

Article Title: Peruvoside, a Cardiac Glycoside, Induces Primitive Myeloid Leukemia Cell Death

doi: 10.3390/molecules21040534

Figure Lengend Snippet: Figure 2. Peruvoside is more effective in inducing apoptosis in human leukemia cells. (a) Flow cytometry analysis of Annexin V (AV) and Propidium Iodide (PI) expression in KG1a and K562 treated with various CGs at different concentrations for 24 h; (b) Graphs show the percentage of early apoptotic cells (AV+PI´) measured in (a) as well as the fold changes in the percentage of AV+PI´

Article Snippet: Human chronic myelogenous leukemia (CML) K562 cell line and human acute myelogenous leukemia (AML) KG1a cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Expressing

Journal: Molecules

Article Title: Peruvoside, a Cardiac Glycoside, Induces Primitive Myeloid Leukemia Cell Death

doi: 10.3390/molecules21040534

Figure Lengend Snippet: Figure 5. Peruvoside treatment did not alter the intracellular Reactive Oxygen Species (ROS) level nor the loss of MMR in KG1a and K562 cells. (a) Both cell lines were treated with 100 nM of Peruvoside or 200 nM of Pyocyanin for 2 h before stained with ROS indicator; (b) Both cell lines were treated with a positive control or 100 nM of Peruvoside for 24 h followed by JC-1 staining. Fluorescence signal was measured and analyzed by flow cytometry. PUV: Peruvoside.

Article Snippet: Human chronic myelogenous leukemia (CML) K562 cell line and human acute myelogenous leukemia (AML) KG1a cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining, Positive Control, Fluorescence, Cytometry

Journal: Molecules

Article Title: Peruvoside, a Cardiac Glycoside, Induces Primitive Myeloid Leukemia Cell Death

doi: 10.3390/molecules21040534

Figure Lengend Snippet: Figure 6. Up-regulation of cyclin-dependent kinase inhibitor 1A in leukemia cells after Peruvoside treatment. KG1a and K562 cells were treated with different CGs for 24 h. RNA was isolated from each sample and cDNA was synthesized for Real-time PCR analyses with gene specific primers using the SYBR green method. Data was normalized to β-actin expression and the relative quantity of gene expression level was calculated by ∆∆Ct method. Changes in expression are presented as fold change relative to DMSO. Dot line indicates DMSO value set as 1. PUV, Peruvoside; DIG, Digitoxin; OUB, Ouabain. n = 3, *, p < 0.05; **, p < 0.01.

Article Snippet: Human chronic myelogenous leukemia (CML) K562 cell line and human acute myelogenous leukemia (AML) KG1a cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Isolation, Synthesized, Real-time Polymerase Chain Reaction, SYBR Green Assay, Expressing, Gene Expression

Journal: Molecules

Article Title: Peruvoside, a Cardiac Glycoside, Induces Primitive Myeloid Leukemia Cell Death

doi: 10.3390/molecules21040534

Figure Lengend Snippet: Figure 7. Peruvoside activates the apoptosis associated Caspase pathway. Western-blotting analysis shows that among other CGs, treatment of Peruvoside (PUV) at 100 nM for 24 h induced the highest level of cleaved Caspase 3 and 8 expression in KG1a. Treatment with Digitoxin (DIG) at 400 nM induced similar level of Caspase 3 and 8 expression as Peruvoside at 100 nM. Peruvoside also induced the expression of cleaved PARP in both K562 and KG1a. PUV, Peruvoside; DIG, Digitoxin; OUB, Ouabain.

Article Snippet: Human chronic myelogenous leukemia (CML) K562 cell line and human acute myelogenous leukemia (AML) KG1a cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Expressing